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81.
目的探讨麻黄加术汤对类风湿性关节炎的作用机制。方法运用Ⅱ型胶原法建立Wistar大鼠类风湿性关节炎模型,采用麻黄加术汤给予干预,以甲氨喋呤片为阳性对照药。将大鼠随机分为空白组、模型组、甲氨喋呤组、麻黄加术汤组。检测大鼠血清炎性细胞因子IL-1β、TNF-α浓度,观察大鼠膝关节滑膜组织病理改变。结果麻黄加术汤可显著降低大鼠血清炎性细胞因子IL-1β、TNF-α的含量,差异具有统计学意义(P<0.01),对CIA大鼠滑膜组织的炎性细胞浸润、纤维组织增生和巨噬样A型细胞有明显抑制作用,差异具有统计学意义(P<0.01)。结论麻黄加术汤治疗类风湿性关节炎的机制可能与降低炎性因子IL-1β、TNF-α的水平,抑制炎性细胞浸润、纤维组织增生和巨噬样A型细胞增生有关。 更多还原 相似文献
82.
利用淋巴细胞杂交瘤技术制备出抗鸡关节炎病毒特异的、具有群反应特性的单克隆抗体,挑选其中AD8株作为一抗建立检测抗原的间接ELISA,该法具有高度的特异性和敏感性,不仅适用于大面积检测的需要,而且可用于该病的早期诊断。 相似文献
83.
84.
AIM: To investigate the effects of rosiglitazone on fibroblast-like synoviocyte (FLS)-induced osteoclastogenesis in rheumatoid arthritis (RA) and the related mechanism. METHODS: RA-FLS were cocultured with peripheral blood monocytes from healthy volunteers in the presence of macrophage colony-stimulating factor (M-CSF) and rosiglitazone. Osteoclasts were assayed by tartrate-resistant acid phosphatase (TRAP) staining. Resorption lacunae area was identified by toluidine blue staining and quantified by image analysis software. The mRNA expression of RANKL and OPG was evaluated by real-time PCR, and the protein levels of RANKL, OPG, p-ERK, p-p38 and p-JNK were measured by Western blot. RESULTS: Compared with control group (without rosiglitazone treatment), rosiglitazone at concentration of 15 μmol/L significantly decreased the number of osteoclasts (P<0.01) and resorption lacunae area (P<0.05). The expression of RANKL at mRNA and protein levels was significantly down-regulated by rosiglitazone at concentration of 15 μmol/L, while the mRNA and protein expression of OPG was up-regulated (P<0.01). Rosiglitazone (15 μmol/L) significantly decreased the protein level of p-ERK (P<0.05), but not the protein level of p-p38 or p-JNK (P>0.05). CONCLUSION: Rosiglitazone inhibits RA-FLS-induced osteoclast formation and its resorption activity by down-regulating RANKL expression and ERK phosphorylation, suggesting that rosiglitazone may inhibit RA osteoclastogenesis and bone resorption. 相似文献
85.
This study was aimed to avoid the dairy cow arthritis,which could caus huge economic losses to cattle farms.Joint effusion was collected from diseased dairy cows,and the pathogens was isolated and cultured using tryptone broth agar followed by morphological,biochemical tests and PCR amplifying 16S rRNA gene fragments and TA cloning and sequencing to identify it.It was found that the bacteria could form beta hemolytic ring on blood AGAR;And it appeared a single individual or short catenation gram-positive by optical microscopy;The results of biochemical identification showed that esculin sesquihydrate,maltose,sucrose,glucose,and hydrogen peroxide enzyme tests all presented positive;It was sensitive to cefazolin,enrofloxacin,ciprofloxacin,ampicillin,streptomycin,chloramphenicol,neomycin and cefepime,while was resistant to tetracycline,erythromycin,doxycycline and amikacin.The nucleic acid homology of this strain with Staphylococcus aureus was 91%,and with the Staphylococcus carnosus was 92%.According to the 16S rRNA gene fragment sequencing results,and the biological characteristics analysis,the initial isolates were identified as similar to Staphylococcus spp bacteria.The results of this study could provide necessary experimental basis for future diagnosis,prevention as well as treatment of arthritis in cattle farm. 相似文献
86.
Identified in France in 1972, this severe goat disease is now well established in goat breeding areas.Natural infection occurs as a contagious agalactia syndrome, the local lesions of which appear after a septicema stage which can lead to the death of animals in poor condition.This fatal evolution is the general rule among kids which die within a few days, after being fed with infectious milk.The experimental disease is easy to reproduce with wild strains of M. capricolum; it allows to study the immune response and also to assess the value of therapeutic agents.The strains isolated in France constitute a very homogeneous group, from a microbiological point of view; compared with reference strains, some serological variations are demonstrable, which can raise difficulties in strain identification.Microbiological diagnosis is fairly easy, but serological diagnosis is not yet standardized and a manifest cross-reaction exists with another pathogenic agent for goats, M. mycoides var. mycoides.Up to now, the epidemiological characteristics of this mycoplasmosis are to a great extent unknown; recovered animals are carriers which allows the disease to be maintained in flocks, but the features of this carrier state (frequency, duration, predisposing factors) are not well known. Screening of such goats remains uncertain.Specific treatment must be carried out with some antibiotics; indications and results are to be discussed. 相似文献
87.
用SPF鸡胚细胞培养,从临床上呈现类似鸡病毒性关节炎症状病鸡的趾屈肌组织中分离出一株病毒,电镜观察,病毒直径约为75nm,无囊膜,呈六边形,双层衣壳,理化检查,病毒对5-氟脱氧尿核苷、pH5.0和乙醚耐受,胰蛋白酶对病毒感染价无影响;核酸电泳分析,病毒由10个核酸片段组成,呈3-3-1-3分布;血清学试验,该病毒与AVAVS_(1133)株呈交叉反应。上述检查结果表明分离的病毒为鸡病毒性关节炎病毒,结合现场病鸡的临床症状和双份血清抗体检测结果,认定该鸡场此次发生的传染病为鸡病毒性关节炎。 相似文献
88.
将鸡病毒性关节炎病毒(AVAV)鸡胚毒J─1株适应于鸡胚细胞和Vero细胞,经连续传代,通过40.5℃、37℃、32℃的交替升降培养温度培养,筛选出1株毒力较弱的克隆株,该克隆株蚀斑大小为2.7~3.1mm。以105.725TCID50注射于1日龄敏感雏鸡的足掌皮下和以104.725~106.725TCID50注射于1日龄敏感雏鸡胸部肌肉内,对雏鸡无致病性。该克隆株在敏感雏鸡内连传3代,无毒力返强现象。这一结果表明,本试验筛选出的AVAV弱毒株对雏鸡具有良好的安全性和遗传稳定性。 相似文献
89.
Advances in surgical treatment of septic arthritis of the distal interphalangeal joint in cattle: a review 总被引:1,自引:1,他引:0
Heppelmann M Kofler J Meyer H Rehage J Starke A 《Veterinary journal (London, England : 1997)》2009,182(2):162-175
This review describes the indications for surgical treatment of distal interphalangeal joint (DIJ) infection in cattle and presents an overview of former surgical methods that have been modified for current use, including arthrotomy, resection of the distal sesamoid bone, resection of the DIJ and digital amputation. The post-operative life span, reasons for culling, pros and cons of claw-preserving techniques versus digital amputation, and pain management in general are also discussed. It would appear that severe claw diseases that result in septic arthritis of the DIJ do not necessarily lead to a reduced life span if cows are adequately treated and thorough aftercare is provided. 相似文献
90.
鸡病毒性关节炎病毒S1133株,在鸡胚成纤维细胞培养传代,至第6代后,细胞产毒效价明显提高,已达到了鸡培养的产毒水平。这为开发细胞培养工艺提供重要依据。 相似文献